Anti-Cyclin-dependent kinase 9 Cdk9 Antibody

Primary Antibodies

42778 MW

WB: 1:1, 000
ICC: 1:50-1:200
IHC: 1:50-1:200

Cyclin-dependent kinase 9

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Protein kinase involved in the regulation of
transcription. Member of the cyclin-dependent kinase pair
(CDK9/cyclin-T) complex, also called positive transcription
elongation factor b (P-TEFb), which facilitates the transition
from abortive to productive elongation by phosphorylating the CTD
(C-terminal domain) of the large subunit of RNA polymerase II
(RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when
in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1,
RPB1/POLR2A and AR, and the negative elongation factors DSIF and
NELF. Regulates cytokine inducible transcription networks by
facilitating promoter recognition of target transcription factors
(e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3
signaling). Promotes RNA synthesis in genetic programs for cell
growth, differentiation and viral pathogenesis. P-TEFb is also
involved in cotranscriptional histone modification, mRNA
processing and mRNA export. Modulates a complex network of
chromatin modifications including histone H2B monoubiquitination
(H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3;
integrates phosphorylation during transcription with chromatin
modifications to control co-transcriptional histone mRNA
processing. The CDK9/cyclin-K complex has also a kinase activity
towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb
in vitro. Replication stress response protein; the CDK9/cyclin-K
complex is required for genome integrity maintenance, by promoting
cell cycle recovery from replication arrest and limiting single-
stranded DNA amount in response to replication stress, thus
reducing the breakdown of stalled replication forks and avoiding
DNA damage. In addition, probable function in DNA repair of
isoform 2 via interaction with KU70/XRCC6. Promotes cardiac
myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD
activates transcription. AR phosphorylation modulates AR
transcription factor promoter selectivity and cell growth. DSIF
and NELF phosphorylation promotes transcription by inhibiting
their negative effect. The phosphorylation of MYOD1 enhances its
transcriptional activity and thus promotes muscle differentiation.

Boster Bio Anti-Cyclin-dependent kinase 9 Cdk9 Antibody catalog # A00794-2. Tested in WB, ICC/IF, IHC, IP applications. This antibody reacts with Human.