Human KLK1 PicoKine ELISA Kit

Manufacturer Boster
Category Products / ELISA
Type Elisa-Kit
Specific against Human
Applications ELISA
Amount 96 Wells
ArtNr BOS-EK0903
Eclass 6.1 32160605
Eclass 9.0 32160605
Available
Gene name
KLK1
Assay range
156pg/ml-10, 000pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of human KLK1 in cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).
Uniprot
P06870
Immunogen
Expression system for standard: NSO; Immunogen sequence: I25-S262
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<10pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).
Anticoagulant
heparin or EDTA
Assay procedure step1
10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml
Background
Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Research category
|cardiovascular|blood|serum proteins| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|kallikreins| cancer|invasion/microenvironment|angiogenesis| cell biology|proteolysis / ubiquitin|proteolytic enzymes|serine protease| kits/ lysates/ other|elisa kits|cardiovascular elisa kits
Synonyms
Kallikrein-1; 3.4.21.35; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein; KLK1;
Gene full name
Kallikrein-1
MW
28890 MW
Protein function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Tissue specificity
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. .
Protein name
Kallikrein-1
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1468|3882|6352|1625|4190|6526
Standard deviation|67.53|260.1|349.4|95.9|301.7|417.7
CV(%)|4.6|6.7|5.5|5.9|7.2|6.4
Sample data
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000
O.D.|0.016|0.084|0.157|0.322|0.513|0.965|1.516|2.020
Tmb Incubation time
25-30min
Principle
monoclonal antibody from mouse|polyclonal antibody from goat
Normal range citations
18627300
Amount: 96 Wells
Available: In stock
Listprice: €381.82
Discount: -10%
Price: €343.64
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Salesmanager: Arne Pelz

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Safety datasheet

BOS-EK0903.pdf

Instruction sheet

BOS-EK0903.pdf