Human IL-29 PicoKine ELISA Kit

Manufacturer Boster
Category Products / ELISA
Type Elisa-Kit
Specific against Human
Applications ELISA
Amount 96 Wells
ArtNr BOS-EK0964
Eclass 6.1 32160605
Eclass 9.0 32160605
Available
Gene name
IFNL1
Assay range
62.5pg/ml-4000pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of human IL-29 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Uniprot
Q8IU54
Immunogen
Expression system for standard: NSO; Immunogen sequence: G20-T200
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<10pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Anticoagulant
heparin or EDTA
Assay procedure step1
4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml
Background
Interferon lambda-1, also called Interleukin-29(IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
Research category
|immunology|innate immunity|cytokines|interleukins
Synonyms
Interferon lambda-1; IFN-lambda-1; Cytokine Zcyto21; Interleukin-29; IL-29; IFNL1; IL29, ZCYTO21;
Gene full name
Interferon lambda-1
MW
21898 MW
Protein function
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN- stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type- selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
Subcellular localization
Secreted.
Protein name
Interferon lambda-1
Sequence similarities
Belongs to the lambda interferon family.
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|517|1446|2336|643|1550|2468
Standard deviation|33.1|76.64|114.5|45.01|105.4|140.7
CV(%)|6.4|5.3|4.9|7|6.8|5.7
Sample data
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000
O.D.|0.051|0.096|0.127|0.207|0.363|0.646|1.115|1.716
Tmb Incubation time
25-30min
Principle
monoclonal antibody from mouse|polyclonal antibody from goat
Normal range citations
23078630
Amount: 96 Wells
Available: In stock
Listprice: €302.73
Discount: -10%
Price: €272.46
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Safety datasheet

BOS-EK0964.pdf

Instruction sheet

BOS-EK0964.pdf