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Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Item no.

BOS-EZ0824

Manufacturer

Boster

Amount

5 plates/kit

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<10pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Oxidized low-density lipoprotein receptor 1;Ox-LDL receptor 1;C-type lectin domain family 8 member A;Lectin-like oxidized LDL receptor 1;LOX-1;Lectin-like oxLDL receptor 1;hLOX-1;Lectin-type oxidized LDL receptor 1;Oxidized low-density lipoprotein receptor 1, soluble form;OLR1;CLEC8A, LOX1;

Available

Manufacturer - Category

EZ-Set™ ELISA Kits (Ab Pairs)

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

30959 MW

Manufacturer - Gene Name

OLR1

Gene Full Name

Oxidized low-density lipoprotein receptor 1

Sample Type

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant human LOX-1/OLR1

Intra Assay Consistency & Inter Assay Consistency

	Intra-Assay Precision			Inter-Assay Precision
Sample
Kit Components	Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard\|
Anticoagulant	heparin or EDTA
Background	LOX-1 (lectin-type oxidized LDL receptor 1) also known as OLR1, is a protein that in humans is encoded by the OLR1 gene. LOX-1 is a receptor protein which belongs to the C-type lectin superfamily. The LOX1 gene is mapped to 12p13-p12 by fluorescence in situ hybridization. LOX1 is expressed on the plasma membrane of differentiated macrophages, but not on monocytes. The LOX1 protein acts as a macrophage scavenger receptor. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.
Immunogen	Expression system for standard: NS0; Immunogen sequence: S61-Q273
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Research Category	Cardiovascular, Cell Biology, Fatty Acids, Lipid Metabolism, Lipids/Lipoproteins, Metabolism, Oxidative Stress, Signal Transduction
Protein Name	Oxidized low-density lipoprotein receptor 1
Protein Function	Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria.
Subcellular Localization	Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted. A secreted form also exists. Localization to membrane rafts requires palmitoylation.
Description	Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human OLR1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Tissue Specificity	Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
Sequence Similarities	Contains 1 C-type lectin domain.

Human LOX-1/OLR1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EZ0824-datasheet.pdf

BOS-EZ0824-safety-datasheet.pdf