Human APOA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

apolipoprotein A-I

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Cardiovascular, Cholesterol Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Secreted.

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.

Concentration (ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200
O.D. | 0.009 | 0.13 | 0.228 | 0.425 | 0.819 | 1.261 | 1.894 | 2.41

Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 4918 | 4239 | 5307 | 3555 | 4505 | 336.96 | 7.5%
Sample 2 | 18666 | 19845 | 16500 | 24262 | 19818 | 400.33 | 2.0%
Sample 3 | 98104 | 76874 | 81171 | 83329 | 84870 | 5236.45 | 6.2%
*number of samples for each test n=16.

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.