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Mouse Resistin PicoKine® Quick ELISA Kit

Mouse Resistin PicoKine® Quick ELISA Kit

Item no.	BOS-FEK0582
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Metabolism Diabetes Glucose Homeostasis Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<2 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Resistin,Resistin,isoform CRA_a,Retn,Xcp4,mCG_13014
Available
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	12492 MW
Gene Name	Retn
Gene Full Name	resistin
Manufacturer - Specificity	Natural and recombinant mouse Resistin
Background	Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: S21-S114
Protein Name	Resistin
Description	Mouse Resistin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Retn in cell culture supernatants, serum and plasma (heparin). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin).
Sample Data	Concentration (pg/ml) \| 0 \| 31.25 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 \| O.D. \| 0.006 \| 0.122 \| 0.245 \| 0.441 \| 0.743 \| 1.101 \| 1.748 \| 2.241
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 77 \| 245 \| 929 \| 80 \| 252 \| 861 Standard deviation \| 6.2%2 \| 13.23 \| 57.59 \| 7.28 \| 17.64 \| 69.74 CV (%) \| 7.7% \| 5.4% \| 6.2% \| 9.1% \| 7% \| 8.1%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 77 \| 78 \| 88 \| 84 \| 81 \| 4.49 \| 5.5% Sample 2 \| 245 \| 263 \| 267 \| 252 \| 256 \| 8.72 \| 3.4% Sample 3 \| 929 \| 803 \| 900 \| 906 \| 884 \| 48.28 \| 5.4% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-FEK0582-datasheet.pdf

BOS-FEK0582-safety-datasheet.pdf