Comparison

ELISA Kit for Claudin 1 (CLDN1)

Manufacturer Cloud-Clone
Category
Type Elisa-Kit
Specific against Human
Applications ELISA
Amount 48T
Item no. SEC388Hu-48
eClass 6.1 32160605
eClass 9.0 32160605
Available
Target Name
Claudin 1
Alternative Names
CLD1, ILVASC, SEMP1, Senescence-Associated Epithelial Membrane Protein 1
Uniprot
O95832
Detection range
0.156-10ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.057ng/mL
Sample type
Tissue homogenates, cell lysates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Claudin 1 (CLDN1).
No significant cross-reactivity or interference between Claudin 1 (CLDN1) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 1 (CLDN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 1 (CLDN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 1 (CLDN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 1 (CLDN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 1 (CLDN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 1 (CLDN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Signal transduction, Tumor immunity,

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

Amount: 48T
Available: In stock
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