Comparison

Recombinant Human 5-formyltetrahydrofolate cyclo-ligase(MTHFS)

Manufacturer Cusabio
Category
Type Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Amount 100ug
Host E.coli
Item no. CSB-EP015159HU-100
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Areas
Metabolism
Uniprot ID
P49914
Gene Names
MTHFS
Organism
Homo sapiens (Human)
AA Sequence
AAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVL SQKVIAHSEYQKSKRISIFLSMQDEIETEEIIKDI FQRGKICFIPRYRFQSNHMDMVRIESPEEISLLPK TSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDK HGNRLGRGKGYYDAYLKRCLQHQEVKPYTLALAFK EQICLQVPVNENDMKVDEVLYEDSSTA
Expression Region
2-203aa
Sequence Info
Full Length of Mature Protein
Source
E.coli
Tag Info
N-terminal 6xHis-SUMO-tagged
MW
39.1 kDa
Alternative Name(s)
5, 10-methenyl-tetrahydrofolate synthetase ; MTHFS ; Methenyl-THF synthetase
Relevance
Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5, 10-methenyltetrahydrofolate.
Reference
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5, 10-methenyltetrahydrofolate.
Subcellular Location
Cytoplasm
Protein Families
5-formyltetrahydrofolate cyclo-ligase family
Tag Information
N-terminal 6xHis-SUMO-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ug
Available: In stock
available

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