Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
100ug |
Host |
E.coli |
Item no. |
CSB-EP015452HU-100 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Research Areas |
Cell Biology |
Uniprot ID |
O96009 |
Gene Names |
NAPSA |
Organism |
Homo sapiens (Human) |
AA Sequence |
KPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTG SSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQAN GTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFG EALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPM DVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGS DPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTL CAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPL LAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYV IQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFL GTYVAVFDRGDMKSSARVGLARARTRGADLGWGET AQAQFPG |
Expression Region |
64-420aa |
Sequence Info |
Full Length of Mature Protein |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-tagged |
MW |
42.5 kDa |
Alternative Name(s) |
Aspartyl protease 4 ; ASP4 ; Asp 4Napsin-1TA01/TA02 |
Relevance |
May be involved in processing of pneumocyte surfactant precursors. |
Reference |
Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity.Yan R., Bienkowski M.J., Shuck M.E., Miao H., Tory M.C., Pauley A.M., Brashier J.R., Stratman N.C., Mathews W.R., Buhl A.E., Carter D.B., Tomasselli A.G., Parodi L.A., Heinrikson R.L., Gurney M.E.Nature 402:533-537(1999) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
May be involved in processing of pneumocyte surfactant precursors. |
Subcellular Location |
Secreted |
Protein Families |
Peptidase A1 family |
Tissue Specificity |
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. |
Tag Information |
N-terminal 6xHis-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.