Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
100ug |
Host |
E.coli |
Item no. |
CSB-EP015802MO-100 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Research Areas |
Others |
Uniprot ID |
P10493 |
Gene Names |
Nid1 |
Organism |
Mus musculus (Mouse) |
AA Sequence |
SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVM NHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFA VEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLK QQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEP YTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHT HIYQWRQTITFQECAHDDARPALPSTQQLSVDSVF VLYNKEERILRYALSNSIGPVRDGSPDA |
Expression Region |
428-665aa |
Sequence Info |
Partial |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-tagged |
MW |
30.3 kDa |
Alternative Name(s) |
Entactin |
Relevance |
Sulfated glycoprotein widely distributed in basent mbranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-Extracellular domain matrix interactions. |
Reference |
Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo.Lee H.K., Seo I.A., Park H.K., Park H.T.FEBS Lett. 580:2253-2257(2006) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. |
Subcellular Location |
Secreted, extracellular space, extracellular matrix, basement membrane |
Tag Information |
N-terminal 6xHis-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.