Comparison

Recombinant Mouse Homeobox protein Nkx-2.2(Nkx2-2)

Manufacturer Cusabio
Category
Type Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Amount 1mg
Host E.coli
Item no. CSB-EP015842MO-1
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Areas
Others
Uniprot ID
P42586
Gene Names
Nkx2-2
Organism
Mus musculus (Mouse)
AA Sequence
MSLTNTKTGFSVKDILDLPDTNDEDGSVAEGPEEE SEGPEPAKRAGPLGQGALDAVQSLPLKSPFYDSSD NPYTRWLASTEGLQYSLHGLAASAPPQDSSSKSPE PSADESPDNDKETQGGGGDAGKKRKRRVLFSKAQT YELERRFRQQRYLSAPEREHLASLIRLTPTQVKIW FQNHRYKMKRARAEKGMEVTPLPSPRRVAVPVLVR DGKPCHALKAQDLAAATFQAGIPFSAYSAQSLQHM QYNAQYSSASTPQYPTAHPLVQAQQWTW
Expression Region
1-273aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal 6xHis-SUMO-tagged
MW
46.1 kDa
Alternative Name(s)
Homeobox protein NK-2 homolog B
Relevance
Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter.
Reference
Regional expression of the homeobox gene Nkx-2.2 in the developing mammalian forebrain.Price M., Lazzaro D., Pohl T., Mattei M.-G., Ruether U., Olivo J.-C., Duboule D., di Lauro R.Neuron 8:241-255(1992)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter.
Subcellular Location
Nucleus
Protein Families
NK-2 homeobox family
Tissue Specificity
Expressed in restricted areas of the developing CNS: the hindbrain and forebrain, and pancreas.
Tag Information
N-terminal 6xHis-SUMO-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1mg
Available: In stock
available

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