Comparison

Recombinant Mouse DNA-dependent protein kinase catalytic subunit(Prkdc),partial

Manufacturer Cusabio
Category
Type Proteins
Specific against other
Amount 1mg
Host E.coli
Item no. CSB-EP018714MO-1
Eclass 6.1 34160400
Eclass 9.0 42020190
Available
Alternative Name(s)
p460
AA Sequence
EYPFLVKGGEDLRQDQRIEQIFEVMNAILSQDAAC SQRNMQLRTYRVVPMTSRLGLIEWIENTMTLKDLL LSNMSQEEKVANNSDPKAPIRDYKDWLMKVSGKSD AGAYVLMYSRANRTETVVAFRRRESQVPPDLLKRA FVKMSTSPEAFLALRSHFASSHALLCISHWLLGIG DRHLNNFMVAMETGSVIGIDFGHAFGSATQFLPVP ELMPFRLTRQFVSLMLPMKETGLMCTVMVHALRAF RSCAGLLTDTMEIFVKEPSFDWKS
Research Topic
Others
Uniprot ID
P97313
Gene Names
Prkdc
Tag Info
N-terminal 6xHis-tagged
Expression Region
3747-4015aa
MW of Fusion Proten
34, 6
Sequence Info
Partial
Relevance
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artis (DCLRE1C). The assbly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism.
Reference
Phosphorylation of the cryptochrome 1 C-terminal tail regulates circadian period length.Gao P., Yoo S.H., Lee K.J., Rosensweig C., Takahashi J.S., Chen B.P., Green C.B.J. Biol. Chem. 288:35277-35286(2013)
Purity
Greater than 90% as determined by SDS-PAGE.
Storage Buffer
Tris-based buffer, 50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Species
Mus musculus (Mouse)
Amount: 1mg
Available: In stock
Listprice: €1,992.57
Price: €1,992.57
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