Comparison

Recombinant Human GTP-binding nuclear protein Ran(RAN)

Manufacturer Cusabio
Category
Type Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Amount 1mg
Host E.coli
Item no. CSB-EP019307HU-1
Conjugate/Tag GST
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Areas
Cell Biology
Uniprot ID
P62826
Gene Names
RAN
Organism
Homo sapiens (Human)
AA Sequence
MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEF EKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQE KFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWH RDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHR KKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLE FVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALP DEDDDL
Expression Region
1-216aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal GST-tagged
MW
51.4 kDa
Alternative Name(s)
Androgen receptor-associated protein 24
GTPase Ran
Ras-like protein TC4
Ras-related nuclear protein
Relevance
GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport.
Reference
Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line.
Drivas G.T., Shih A., Coutavas E., Rush M.G., D'Eustachio P.
Mol. Cell. Biol. 10:1793-1798(1990)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport.
Subcellular Location
Nucleus, Nucleus envelope, Cytoplasm, Melanosome
Protein Families
Small GTPase superfamily, Ran family
Tissue Specificity
Expressed in a variety of tissues.
Tag Information
N-terminal GST-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1mg
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close