Comparison

Recombinant Human GTP-binding nuclear protein Ran(RAN)

Manufacturer Cusabio
Category
Type Proteins
Specific against other
Amount 500ug
Host E.coli
Item no. CSB-EP019307HU-500
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Topic
Cell Biology
Uniprot ID
P62826
Gene Names
RAN
Organism
Homo sapiens (Human)
AA Sequence
AAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFE KKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEK FGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHR DLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRK KNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEF VAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPD EDDDL
Expression Region
1-216aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal GST-tagged
MW
51.3 kDa
Alternative Name
Androgen receptor-associated protein 24
GTPase Ran
Ras-like protein TC4
Ras-related nuclear protein
Relevance
GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport.
Reference
"Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line."
Drivas G.T., Shih A., Coutavas E., Rush M.G., D'Eustachio P.
Mol. Cell. Biol. 10:1793-1798(1990)
Purity
Greater than 90% as determined by SDS-PAGE.
Storage Buffer
Tris-based buffer, 50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport.
Subcellular Location
Nucleus, Nucleus envelope, Cytoplasm, Melanosome
Protein Families
Small GTPase superfamily, Ran family
Tissue Specificity
Expressed in a variety of tissues.
Amount: 500ug
Available: In stock
Listprice: €1,013.82
Price: €1,013.82
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