Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
10ug |
Host |
E.coli |
Item no. |
CSB-EP019652HU-10 |
Conjugate/Tag |
GST |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Research Topic |
Signal Transduction |
Uniprot ID |
O76081 |
Gene Names |
RGS20 |
Organism |
Homo sapiens (Human) |
AA Sequence |
EPAGASSPAGRVDGGLQMGSERMEMRKRQMPAAQD TPGAAPGQPGAGSRGSNACCFCWCCCCSCSCLTVR NQEDQRPTIASHELRADLPTWEESPAPTLEEVNAW AQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMA CEELKKEANKNIIEEKARIIYEDYISILSPKEVSL DSRVREVINRNMVEPSQHIFDDAQLQIYTLMHRDS YPRFMNSAVYKDLLQSLSEKSIEA |
Expression Region |
1-234aa |
Sequence Info |
Full Length of BC015614 |
Source |
E.coli |
Tag Info |
N-terminal GST-tagged |
MW |
53.4 kDa |
Alternative Name(s) |
Gz-selective GTPase-activating protein |
Relevance |
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins |
Reference |
"RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily." Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M. J. Biol. Chem. 273:26014-26025(1998) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Storage Buffer |
Tris-based buffer, 50% glycerol |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity). |
Subcellular Location |
Membrane, Lipid-anchor, Nucleus, Cytoplasm |
Tissue Specificity |
Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe. |
Tag Information |
N-terminal GST-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.