Comparison

Recombinant Human Receptor-interacting serine/threonine-protein kinase 2(RIPK2)

Manufacturer Cusabio
Category
Type Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Amount 10ug
Host E.coli
Item no. CSB-EP019736HU-10
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Topic
Immunology
Uniprot ID
O43353
Gene Names
RIPK2
Organism
Homo sapiens (Human)
AA Sequence
MNGEAICSALPTIPYHKLADLRYLSRGASGTVSSA RHADWRVQVAVKHLHIHTPLLDSERKDVLREAEIL HKARFSYILPILGICNEPEFLGIVTEYMPNGSLNE LLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTP PLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSL SQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKH DIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQG HRPVINEESLPYDIPHRARMISLIESGWAQNPDER PSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQ SVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQ LHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKL HHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIIN PLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEAC LNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQL LDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILV VSRSPSLNLLQNKSM
Expression Region
1-540aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal 6xHis-tagged
MW
65.2 kDa
Alternative Name(s)
CARD-containing interleukin-1 beta-converting enzyme-associated kinase ; CARD-containing IL-1 beta ICE-kinaseRIP-like-interacting CLARP kinaseReceptor-interacting protein 2 ; RIP-2Tyrosine-protein kinase RIPK2 (EC:2.7.10.2)
Relevance
Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagent of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Reference
Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.Mol. Cell 31:438-448(2008)
Purity
Greater than 90% as determined by SDS-PAGE.
Storage Buffer
Tris-based buffer, 50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Subcellular Location
Cytoplasm
Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity
Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Paythway
NOD-likereceptorsignalingpathway
Tag Information
N-terminal 6xHis-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 10ug
Available: In stock
available

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