Comparison

Recombinant Human RING finger protein 212(RNF212)

Manufacturer Cusabio
Category
Type Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Amount 100ug
Host E.coli
Item no. CSB-EP019872HU-100
Conjugate/Tag GST
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Areas
Epigenetics and Nuclear Signaling
Uniprot ID
Q495C1
Gene Names
RNF212
Organism
Homo sapiens (Human)
AA Sequence
MANWVFCNRCFQPPHRTSCFSLTNCGHVYCDACLG KGKKNECLICKAPCRTVLLSKHTDADIQAFFMSID SLCKKYSRETSQILEFQEKHRKRLLAFYREKISRL EESLRKSVLQIEQLQSMRSSQQTAFSTIKSSVSTK PHGCLLPPHSSAPDRLESMEVDLSPSPIRKSEIAA GPARISMISPPQDGRMGPHLTASFCFIPWLTLSKP PVPGECVISRGSPCFCIDVCPHWLLLLAFSSGRHG ELTNSKTLPIYAEVQRAVLFPFQQAEGTLDTFRTP AVSVVFPLCQFERKKSF
Expression Region
1-297aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal GST-tagged
MW
60.4 kDa
Alternative Name(s)
RING finger protein 212
Relevance
SUMO E3 ligase that acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May mediate sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement and plays an antagonist role with CCNB1IP1/HEI10 in the regulation of meiotic recombination
Reference
Targeted gene knockout reveals a role in meiotic recombination for ZHP-3, a Zip3-related protein in Caenorhabditis elegans.
Jantsch V., Pasierbek P., Mueller M.M., Schweizer D., Jantsch M., Loidl J.
Mol. Cell. Biol. 24:7998-8006(2004)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
SUMO E3 ligase that acts as a regulator of crossing-over during meiosis
Subcellular Location
Nucleus, Chromosome
Tag Information
N-terminal GST-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ug
Available: In stock
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