Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
1mg |
Host |
E.coli |
Item no. |
CSB-EP020443MO-1 |
Conjugate/Tag |
Myc |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
AA Sequence |
AVQISKKRKFVADGIFKAELNEFLTRELAEDGYSG VEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTA VVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLR YKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGK LRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVL LRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIV EPKDEILPTTPISEQKGGKPEPPAMPQPVPTA |
Research Topic |
Others |
Uniprot ID |
P23396 |
Gene Names |
RPS3 |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
Expression Region |
2-243aa |
MW of Fusion Proten |
42, 6 |
Sequence Info |
Full Length |
Relevance |
Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7, 8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535). Represses its own translation by binding to its cognate mRNA (PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408). |
Reference |
"Isolation of a cDNA encoding human 40S ribosomal protein s3."Zhang X.T., Tan Y.M., Tan Y.H.Nucleic Acids Res. 18:6689-6689(1990) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Storage Buffer |
Tris-based buffer, 50% glycerol |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Species |
Mus musculus (Mouse) |
Tag Information |
N-terminal 10xHis-tagged and C-terminal Myc-tagged |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.