Comparison

Recombinant Human Proteasome subunit beta type-4(PSMB4)

Manufacturer Cusabio
Category
Type Proteins
Specific against other
Amount 200ug
Host E.coli
Item no. CSB-RP013744h-200
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Topic
Immunology
Uniprot ID
P28070
Gene Names
PSMB4
Organism
Homo sapiens (Human)
AA Sequence
TQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLAR FRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQM VIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNP LWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATG YGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRV LYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWD IAHMISGFE
Expression Region
46-264aa
Sequence Info
Full Length of Mature Protein
Source
E.coli
Tag Info
N-terminal GST-tagged
MW
51.4 kDa
Alternative Name
26KDA prosomal protein . HsBPROS26 . PROS-26Macropain beta chain. Multicatalytic endopeptidase complex beta chain. Proteasome beta chain. Proteasome chain 3 . HsN3
Relevance
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome . SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.1 Publication
Reference
Sequence analyses and inter-species comparisons of three novel human proteasomal subunits, HsN3, HsC7-I and HsC10-II, confine potential proteolytic active-site residues.Nothwang H.G., Tamura T., Tanaka K., Ichihara A.Biochim. Biophys. Acta 1219:361-368(1994)
Purity
Greater than 90% as determined by SDS-PAGE.
Storage Buffer
Tris-based buffer, 50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Subcellular Location
Cytoplasm, Nucleus
Protein Families
Peptidase T1B family
Amount: 200ug
Available: In stock
Listprice: €628.34
Price: €628.34
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