Recombinant Rat Pyruvate kinase PKM(Pkm)

Manufacturer Cusabio
Category
Type Proteins
Specific against Rat
Amount 50ug
Host E.coli
ArtNr CSB-EP018072RA-50
Eclass 6.1 34160400
Eclass 9.0 42020190
Available
Research Topic
Signal Transduction
Uniprot ID
P11980
Gene Names
Pkm
Organism
Rattus norvegicus (Rat)
AA Sequence
MPKPDSEAGTAFIQTQQLHAAMADTFLEHMCRLDI DSAPITARNTGIICTIGPASRSVEMLKEMIKSGMN VARLNFSHGTHEYHAETIKNVRAATESFASDPILY RPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATL KITLDNAYMEKCDENILWLDYKNICKVVEVGSKIY VDDGLISLQVKEKGADYLVTEVENGGSLGSKKGVN LPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFI RKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRF DEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIG RCNRAGKPVICATQMLESMIKKPRPTRAEGSDVAN AVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREA EAAVFHRLLFEELARASSQSTDPLEAMAMGSVEAS YKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTR NPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRV NLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTM RVVPVP
Expression Region
1-531aa
Sequence Info
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
MW
62.8 kDa
Alternative Name(s)
Pyruvate kinase muscle isozyme
Pkm2, Pykm
Relevance
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation
Reference
"The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing."
Noguchi T., Inoue H., Tanaka T.
J. Biol. Chem. 261:13807-13812(1986)
Purity
Greater than 85% as determined by SDS-PAGE.
Storage Buffer
Tris-based buffer, 50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Amount: 50ug
Available: In stock
Listprice: €342.86
Price: €342.86
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