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Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit

Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit

Item no.	BOS-FEK0773
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology Neuroscience Metabolism Diabetes Molecular Targets for Neuroscience Aging & Neurological Disorders By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	HAI-2/SPINT2\|HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2
Available
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Gene Name	CST3
Gene Full Name	serine peptidase inhibitor, Kunitz type, 2
Manufacturer - Specificity	Natural and recombinant Human SPINT2
Background	Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen	Expression system for standard: NS0; Immunogen sequence: M1-K197
Manufacturer - Research Category	Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
Protein Name	Kunitz-type protease inhibitor 2
Description	Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SPINT2 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates
Sample Data	Concentration (pg/ml) \| 0 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 O.D. \| 0.096 \| 0.157 \| 0.202 \| 0.298 \| 0.473 \| 0.787 \| 1.322 \| 2.105
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 59 \| 211 \| 923 \| 61 \| 224 \| 890 Standard deviation \| 3.07 \| 10.97 \| 61.84 \| 3.54 \| 12.32 \| 62.3 CV (%) \| 5.2% \| 5.2% \| 6.7% \| 5.8% \| 5.5% \| 7%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 59 \| 51 \| 45 \| 47 \| 51 \| 2.22 \| 4.4% Sample 2 \| 211 \| 221 \| 223 \| 199 \| 214 \| 8.33 \| 3.9% Sample 3 \| 923 \| 961 \| 826 \| 917 \| 907 \| 53.5 \| 5.9% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity\|Volume Anti-tag Pre-coated 96-well Strip Microplate\|1\|12 strips of 8 wells Standard\|\|2 Antibody A\|1\|3ml Antibody B\|1\|3ml Sample Diluent\|1\|15ml TBS-T Wash Buffer (25x)\|1\|12ml Color Developing Reagent (TMB)\|1\|10ml Stop Solution\|1\|10ml Adhesive Plate Sealers\|2\|Piece
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Alias	HAI-2/SPINT2, HAI2, HAI-2, HAI-2DIAR3, HAI2FLJ45571, Hepatocyte growth factor activator inhibitor type 2, Kop, kunitz-type protease inhibitor 2, PB, Placental bikunin, serine peptidase inhibitor, Kunitz type, 2, serine protease inhibitor, Kunitz type, 2
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-FEK0773-datasheet.pdf

BOS-FEK0773-safety-datasheet.pdf