Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit

Quick ELISA Kits

RARRES2

Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human Chemerin/RARRES2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1, 250 | 2, 500 | 5, 000
O.D. | 0.013 | 0.119 | 0.195 | 0.356 | 0.623 | 1.012 | 1.389 | 1.802

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 118 | 122 | 103 | 115 | 115 | 6.76 | 5.9%
Sample 2 | 754 | 766 | 687 | 729 | 734 | 27.16 | 3.7%
Sample 3 | 3266 | 2789 | 2969 | 3381 | 3101 | 192.28 | 6.2%
*number of samples for each test n=16.

Description|Quantity|Volume
Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
Standard||2
Antibody A|1|3ml
Antibody B|1|3ml
Sample Diluent|1|15ml
TBS-T Wash Buffer (25x)|1|12ml
Color Developing Reagent (TMB)|1|10ml
Stop Solution|1|10ml
Adhesive Plate Sealers|2|Piece

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.