Human APOE PicoKine® Quick ELISA Kit

Quick ELISA Kits

APOE

Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-absorbance lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low absorbance lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.

Human APOE PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APOE in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, milk and urine. Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200
O.D. | 0.003 | 0.143 | 0.256 | 0.448 | 0.731 | 1.041 | 1.413 | 1.650

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 7336 | 7271 | 6566 | 6791 | 6991 | 265.65 | 3.8%
Sample 2 | 30862 | 28096 | 32231 | 27122 | 29577 | 1449.27 | 4.9%
Sample 3 | 101167 | 109927 | 102578 | 89024 | 100674 | 6241.78 | 6.2%
*number of samples for each test n=16.

Description|Quantity|Volume
Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
Standard||2
Antibody A|1|3ml
Antibody B|1|3ml
Sample Diluent|1|15ml
TBS-T Wash Buffer (25x)|1|12ml
Color Developing Reagent (TMB)|1|10ml
Stop Solution|1|10ml
Adhesive Plate Sealers|2|Piece

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.