GRIN2A ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

glutamate ionotropic receptor NMDA type subunit 2A

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:8768735, PubMed:26919761, PubMed:26875626, PubMed:28105280). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B (PubMed:26919761, PubMed:26875626). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity).

NP_000824.1

Component: Amount
GRIN2A Microplate: 96 Wells (12 x 8 Well strips)GRIN2A Lyophilized Standard: 2100X GRIN2A HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL

2h, 30min

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody (Anti-GRIN2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody (Anti-GRIN2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%