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CD59 ELISA Kit (Human)

CD59 ELISA Kit (Human)

Item no.	OKCD07331
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Neuroinflammation ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 5.5pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	16.3A5;1F5;1F5 antigen;20 kDa homologous restriction factor;CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5,EJ16,EJ30,EL32 and G344);CD59 blood group antigen;CD59 glycoprotein;CD59 molecule,complement regulatory protein;EJ16;EJ30;EL32;G344;HRF20;HRF-20;human leukocyte antigen MIC11;Ly-6-like protein;lymphocytic antigen CD59/MEM43;MACIF;MAC-inhibitory protein;MAC-IP;MEM43;MEM43 antigen;membrane attack complex (MAC) inhibition factor;membrane attack complex inhibition factor;membrane inhibitor of reactive lysis;MIC11;MIN1;MIN2;MIN3;MIRL;MSK21;p18-20;protectin;surface anitgen recognized by monoclonal antibody 16.3A5;T cell-activating protein.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CD59
Gene Fullname	CD59 molecule (CD59 blood group)
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Nucleotide accession_num	NM_000611.5
Protein accession_num	NP_000602.1
Protein name	CD59 glycoprotein
Kit Components	Component: Amount CD59 Microplate: 96 Wells (12 x 8 Well strips)CD59 Lyophilized Standard: 2100X CD59 HRP-Detector Anitbody: 1 x 120 Ã‚uL100X Avidin-HRP Conjugate: 1 x 120 Ã‚uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster of Differentiation 59 (CD59). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster of Differentiation 59 (CD59). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster of Differentiation 59 (CD59), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster of Differentiation 59 (CD59) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD07331.pdf

OKCD07331-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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