Comparison

GP1BA ELISA Kit (Human)

Item no. OKCD07618
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity < 7.5pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias antigen CD42b-alpha;BDPLT1;BDPLT3;BSS;CD42B;CD42b-alpha;DBPLT3;glycoprotein Ib (platelet),alpha polypeptide;glycoprotein Ib platelet alpha subunit;GP1B;GP-Ib alpha;GPIbA;GPIbalpha;mutant platelet membrane glycoprotein Ib-alpha;platelet glycoprotein Ib alpha chain;platelet membrane glycoprotein 1b-alpha subunit;platelet membrane glycoprotein Ib-alpha;VWDP.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
GP1BA
Gene Fullname
glycoprotein Ib platelet subunit alpha
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
Nucleotide accession_num
NM_000173.6
Protein accession_num
NP_000164.5
Protein name
Platelet glycoprotein Ib alpha chain
Kit Components
Component: Amount
GP1BA Microplate: 96 Wells (12 x 8 Well strips)GP1BA Lyophilized Standard: 2100X GP1BA HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection
Colorimetric
Kit duration
3h
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycocalicin (GC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycocalicin (GC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycocalicin (GC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycocalicin (GC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycocalicin (GC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycocalicin (GC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Plasma

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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