RelA p65 NF-kappa B (NF-kB, Nuclear Factor-kappa B) ELISA Assay Kit, Rat Specificity ( NFKB-ELISA (r, 96T)

Manufacturer Fivephoton
Category Products / ELISA
Type Elisa-Kit
Specific against other
Amount 96T
Eclass 6.1 32160605
Eclass 9.0 32160605
Measures total RelA(p65) in sandwich ELISA format
Affinity purified polyclonal capture antibody with polyclonal HRP-conjugated detection antibody
Strip plates
p65 Standard Concentration: 2700pg/ml
Assay Range: 30pg/ml - 1800pg/ml
HRP-colorimetric detection
Rapid Assay: Total incubation time with plate and HRP-detection antibody 1hr at 37C
Nuclear-cytoplasmic isolation kit is offered to quantify nuclear p65 (see available options menu).
The RelA p65 NF-kB (NF-kappa B, NF-{kappa}B) ELISA kit employs the sandwich ELISA(96T) format to quantitate NF-kB p65 protein levels. The 96-well strip plate is coated with an affinity purified polyclonal capture antibody selective to the RelA (p65) subunit of NF-kB. After addition of sample, incubation and wash, a second polyclonal anti-RelA (p65) HRP-conjugated antibody is added to each well. Color reagents are added to generate a colorimetric reaction, which corresponds to the concentration of RelA (p65) in the sample.

A Nuclear-Cytoplasmic fractionation kit is offered to partition cytoplasmic and nuclear p65. p65(RelA) in the nuclear and cytoplosmic fractions can be directly measured with this kit to provide an estimate of NF-kappa B activation by calculation of the nuclear p65 relative to cytoplasmic p65 concentration ratio.
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Amount: 96T
Available: In stock
Listprice: €817.80
Price: €817.80

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