ECL Chemiluminescence High Sensitivity Western Blot Detection Kit (PCD-250 and PCD-500)

Manufacturer Fivephoton
Category Products / Kits
Type Kit
Specific against other
Amount 1 kit
ArtNr PCD-250
Eclass 6.1 32161000
Eclass 9.0 32161090
Available
Highlights
Strong signal ECL Western blotting protein detection method
Sensitive picogram detection
Applicable to PVDF or nitrocellulose membranes. Develop on film or CCD camera
Effective tool for publication quality Western Blot figures
Background
ECL Chemiluminescence Western Blot Detection (PCD) Kit Overview
The horseradish peroxidase (HRP)-catalyzed enhanced chemiluminescence method is commonly known as the ECL detection method for Western blots. Proteins in Western blots are recognized by specific primary antibodies, which are in turn detected by a secondary antibodies coupled to horse-radish peroxidase (HRP). In the ECL reaction, HRP catalyzes luminol oxidation in the presence of peroxidase and enhancer. The resulting luminescence that corresponds to protein bands in Western blots is detected on film or by a CCD camera.

ECL Chemiluminescence Detection Kit: Protocol Synopsis
To detect protein bands transferred to PVDF or nitrocellulose membrane, premix solution A, which contains the peroxidase substrate, luminol and enhancer, with the peroxide solution B. Incubate the membrane with the premixed solution for one minute. Bright luminescence will be emitted that can be captured on film or a CCD camera.
Description
The Photon ECL Chemiluminescence Western Blotting Detection Kit (PCD) contains reagents to rapidly and reliably detect proteins in Western blots to picogram protein amounts. The unique feature of this ECL reagent kit is its high signal to noise luminescence, providing outstanding selectivity and robust signal.
Amount: 1 kit
Available: In stock
Listprice: €231.75
Price: €231.75
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