Antibodies

MOUSE ANTI HUMAN CYTOCHROME B245 LIGHT CHAIN

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against Human
Applications WB, IP
Amount 0.1 mg
Host Mouse
ArtNr 20-783-314809
Available
Genway ID:
GWB-82309D
Specificity:
CYTOCHROME B245 LIGHT CHAIN
Isotype:
IgG1
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09% Sodium Azide (NaN3)Approx. Protein Concentrations: IgG concentration 1. 0mg/ml
Immunogen:
Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
Specificity:
Recognises the light chain of cytochrome B245 also known as p22phox a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease characterised by severe recurrent infections. The light chain (p22phox) along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b or Cyt b) which is always membrane-resident. Upon the appropriate stimulus other components of NADPH oxidase (p47phox p40phox p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE) a cytosolic epitope on p22phox not accessible on intact neutrophils. Clone CS9 also has an inhibitory effect on NADPH oxidase activation. Recommended Negative Controls: MOUSE IgG1 NEGATIVE CONTROLRecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)
Amount: 0.1 mg
Available: In stock
Listprice: €326.19
Price: €326.19
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