MOUSE ANTI HUMAN CD59

GWB-32C451

IgG2a

Phosphate buffered saline

Reacts with a PI-linked glycoprotein Mw 18-20kD found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. MEM-43 has been shown to stimulate NK activity. CD59 is expressed by erythrocytes and therefore staining in whole blood systems requires a high concentration of antibody. * The epitope recognised by MEM-43 is lost after reduction therefore non-reducing conditions are required for western blotting techniques (2 5). Recommended Negative Controls: MOUSE IgG2a NEGATIVE CONTROLRecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGHuCAL Anti Mouse IgG2aRabbit Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMGoat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)

Soluble form found in a number of tissues. Ref. 12Ref. 16Ref. 18Ref. 19Ref. 20Post-translational modificationN- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide Gal-beta-1 3GalNAc and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Ref. 16Ref. 11Ref. 21Glycated. Glycation is found in diabetic subjects but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. Involvement in diseaseDefects in CD59 are the cause of CD59 deficiency [MIM:612300]. Ref. 25Sequence similaritiesContains 1 UPAR/Ly6 domain.