MOUSE ANTI HUMAN CD8:FITC

GWB-54C84C

IgG1Species Cross Reactivity: Reacts with: Rhesus MonkeyN. B. Antibody reactivity and working conditions may vary between species.

Phosphate buffered saline pH7. 4

Normal human blood lymphocytes

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit structureIn general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref. 7Subcellular locationIsoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Post-translational modificationAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers while the four N-terminal cysteines do not By similarity. Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells leading to recurrent bacterial infections. Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Bellingan. G. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J. Exp. Med. 196:1515-1521.