Antibodies

RAT ANTI MOUSE CD8 ALPHA:FITC

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against Mouse
Amount 0.1 mg
Host Rat
ArtNr 20-783-314691
Available
Genway ID:
GWB-3AD31C
Specificity:
CD8 ALPHA
Isotype:
IgG2a
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 2
Preservative Stabilisers:
0. 09% Sodium Azide (NaN3) 1% Bovine Serum AlbuminApprox. Protein Concentrations: IgG concentration 0. 5mg/ml
Immunogen:
Mouse thymus or spleen.
Specificity:
Detects mouse CD8a the alpha chain of the CD8 antigen. CD8 is expressed on T-cells and exists either a homodimer composed of two alpha chains or a heterodimer composed of one alpha and one beta chain. CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules. The 53-6. 7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Recommended Negative Controls: RAT IgG2a NEGATIVE CONTROL:FITC
Function:
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit structureIn general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Subcellular locationMembrane; Single-pass type I membrane protein. Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain. [1] \" Cloning and characterization of platelet factor 4 cDNA derived from a human erythroleukemic cell line. \" Poncz M. Surrey S. Larocco P. Weiss M. J. Rappaport E. F. Conway T. M. Schwartz E. Blood 69:219-223(1987) [PubMed: 3098319] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] \" Structural and functional comparison of the genes for human platelet factor 4 and PF4alt. \" Eisman R. Surrey S. Ramachandran B. Schwartz E. Poncz M. Blood 76:336-344(1990) [PubMed: 1695112] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [3] \" Localization of distal regulatory domains in the megakaryocyte-specific platelet basic protein/platelet factor 4 gene locus. \" Zhang C. Thornton M. A. Kowalska M. A. Sachis B. S. Feldman M. Poncz M. McKenzie S. E. Reilly M. P. Blood 98:610-617(2001) [PubMed: 11468158] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [4] \" Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). \" Ebert L. Schick M. Neubert P. Schatten R. Henze S. Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [5] \" Generation and annotation of the DNA sequences of human chromosomes 2 and 4. \" Hillier L. W. Graves T. A. Fulton R. S. Fulton L. A. Pepin K. H. Minx P. Wagner-McPherson C. Layman D. Wylie K. Sekhon M. Becker M. C. Fewell G. A. Delehaunty K. D. Miner T. L. Nash W. E. Kremitzki C. Oddy L. Du H. Sun H. Bradshaw-Cordum H. Ali J. Carter J. Cordes M. Harris A. Isak A. van Brunt A. Nguyen C. Du F. Courtney L. Kalicki J. Ozersky P. Abbott S. Armstrong J. Belter E. A. Caruso L. Cedroni M. Cotton M. Davidson T. Desai A. Elliott G. Erb T. Fronick C. Gaige T. Haakenson W. Haglund K. Holmes A. Harkins R. Kim K. Kruchowski S. S. Strong C. M. Grewal N. Goyea E. Hou S. Levy A. Martinka S. Mead K. McLellan M. D. Meyer R. Randall-Maher J. Tomlinson C. Dauphin-Kohlberg S. Kozlowicz-Reilly A. Shah N. Swearengen-Shahid S. Snider J. Strong J. T. Thompson J. Yoakum M. Leonard S. Pearman C. Trani L. Radionenko M. Waligorski J. E. Wang C. Rock S. M. Tin-Wollam A. -M. Maupin R. Latreille P. Wendl M. C. Yang S. -P. Pohl C. Wallis J. W. Spieth J. Bieri T. A. Berkowicz N. Nelson J. O. Osborne J. Ding L. Meyer R. Sabo A. Shotland Y. Sinha P. Wohldmann P. E. Cook L. L. Hickenbotham M. T. Eldred J. Williams D. Jones T. A. She X. Ciccarelli F. D. Izaurralde E. Taylor J. Schmutz J. Myers R. M. Cox D. R. Huang X. McPherson J. D. Mardis E. R. Clifton S. W. Warren W. C. Chinwalla A. T. Eddy S. R. Marra M. A. Ovcharenko I. Furey T. S. Miller W. Eichler E. E. Bork P. Suyama M. Torrents D. Waterston R. H. Wilson R. K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. [6] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [7] \" Isolation crystallization and primary amino acid sequence of human platelet factor 4. \" Hermodson M. Schmer G. Kurachi K. J. Biol. Chem. 252:6276-6279(1977) [PubMed: 893407] [Abstract]Cited for: PROTEIN SEQUENCE OF 32-101. [8] \" Amino acid sequence of human platelet factor 4. \" Deuel T. F. Keim P. S. Farmer M. Heinrikson R. L. Proc. Natl. Acad. Sci. U. S. A. 74:2256-2258(1977) [PubMed: 267922] [Abstract]Cited for: PROTEIN SEQUENCE OF 32-101. [9] \" Primary structure of human platelet factor 4. \" Walz D. A. Wu V. Y. de Lamo R. Dene H. McCoy L. E. Thromb. Res. 11:893-898(1977) [PubMed: 601757] [Abstract]Cited for: PROTEIN SEQUENCE OF 32-101. [10] \" Complete covalent structure of human platelet factor 4. \" Morgan F. J. Begg G. S. Chesterman C. N. Thromb. Haemost. 42:1652-1660(1980) [PubMed: 6445090] [Abstract]Cited for: PROTEIN SEQUENCE OF 32-101.
Amount: 0.1 mg
Available: In stock
Listprice: €212.88
Price: €212.88
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