CD45

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone YW62. 3
Applications FC
Amount 0.025 mg
ArtNr 20-783-310011
Available
Genway ID:
GWB-57BBF0
Specificity:
CD45NCBI
Gene ID:
19264
Specificity:
CD45
Clone:
YW62. 3
Immunogen:
Mouse spleen cells.
Fusion Partner:
Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1. 2. 3 myeloma cell line.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline
Preservative Stabilisers:
0. 09%Sodium Azide1%Bovine Serum AlbuminSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (/). Suggested
Dilution:
Flow Cytometry - Neat
Function:
Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation recruits an dephosphorylates SKAP1 and FYN.
Catalytic Activity:
Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit:
Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Domain:
The first PTPase domain interacts with SKAP1.
Ptm:
Heavily N- and O-glycosylated.
Disease:
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Disease:
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Similarity:
Contains 2 fibronectin type-III domains.
Similarity:
Contains 2 tyrosine-protein phosphatase domains. 1. Watt. S. M. et al. (1987) Cell surface markers on haemapoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations. 2. Bindon. C. I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies.
Amount: 0.025 mg
Available: In stock
Listprice: €168.20
Discount: -10.0%
Price: €151.38
You save: €16.82
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