CD247


Manufacturer	GENWAY
Category	Oncology Antibodies Flow Cytometry Immunoprecipitation Western Blot ELISA Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody
Specific against	other
Clone	G3
Applications	WB, FC, IP, ELISA
Amount	0.025 mg
Item no.	20-783-310050
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-AD5CF5
Specificity:	CD247NCBI
Gene ID:	919
Specificity:	CD247
Clone:	G3
Immunogen:	113 amino acid synthetic peptide corresponding to the cytoplasmic domain of murine zeta TCR subunit.
Fusion Partner:	Spleen cells from immunised BALB/c mice were fused with cells of the Ag8. 653 mouse myeloma cell line.
Specificity Note:	The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events. A novel monoclonal antibody named G3 has been developed that is specific for the TCR zeta chain subunit (CD247). The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain.
Preparation:	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline pH7. 2
Preservative Stabilisers:		0. 09%Sodium AzideSuggested
Flow Cytometry:	Membrane permeabilisation is required for this application using a digitonin based method. Suggested
Dilution:	Flow Cytometry - 1/50 - 1/100Immunohistology - Resin - 1ug/mlImmunoprecipitation - 2ug/ml
Function:	Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Subunit:	The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma delta epsilon zeta and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts with DOCK2 and TRAT1. Interacts with HIV-1 Nef protein. Interacts with SHB.
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Domain:	The ITAM domains mediate interaction with SHB.
Ptm:	Phosphorylated on Tyr residues after T-cell receptor triggering (By similarity).
Disease:	Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.
Similarity:	Belongs to the CD3Z/FCER1G family.
Similarity:	Contains 3 ITAM domains. 1. van Oers. N. S. C. et al. (1994) Production and characterisation of monoclonal antibodies specific for the murine T cell receptor zeta chain. 2. Anderson. P. (1995) mAb reative with lymphocyte restricted intracellular antigens. Leucocyte Typing V p325. Oxford University Press.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 0.025 mg

available

Delivery expected until 5/9/2024