Manufacturer |
GENWAY
|
Category |
|
Type |
Antibody |
Specific against |
other |
Clone |
B199. 2 |
Applications |
FC, IP, IHC |
Amount |
0.025 mg |
Item no. |
20-783-310178 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Genway ID: |
GWB-BD520A |
Description: |
MOUSE ANTI HUMAN CD161 |
Specificity: |
MCA1855 recognises the hyman CD161 cell surface antigen expressed by almost all NK cells and with a small subset of CD3+ve T cells. CD161 is a homodimeric cell surface protein comprising two chains of 40-44kD. Clone B199. 2 recognises a similar epitope to the DX9 monoclonal antibody. |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 106cells in 100ul. Method sheets are available on request. NCBI |
Gene ID: |
3820 |
Isotype: |
CD161 |
Clone: |
B199. 2 |
Fusion Partner: |
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63. Ag8. 653 myeloma cell line. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution: |
Phosphate buffered saline |
|
Preservative Stabilisers: |
| 0. 09%Sodium AzideSuggested |
Dilution: |
Flow Cytometry - 1/50 - 1/100 |
Function: |
Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1 3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. |
Subunit: |
Homodimer; disulfide-linked. Interacts with acid sphingomyelinase/SMPD1. |
Subcellular Location: |
Membrane; Single-pass type II membrane protein (Potential). |
Tissue Specificity: |
Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. |
Induction: |
By IL12 in NK cells. |
Ptm: |
N-glycosylated. Contains sialic acid residues. |
Similarity: |
Contains 1 C-type lectin domain. 1. Bennett. I. M. et al. (1996) Definition of natural killer NKR-PIA+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. 2. Azzoni. L. et al. (1998) Differential transcriptional regulation of CD161 and a novel gene. 197/15a. by IL-2. IL-15. and IL-12 in NK and T cells. |
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