CD161

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone B199. 2
Applications FC, IP, IHC
Amount 0.025 mg
ArtNr 20-783-310178
Available
Genway ID:
GWB-BD520A
Description:
MOUSE ANTI HUMAN CD161
Specificity:
MCA1855 recognises the hyman CD161 cell surface antigen expressed by almost all NK cells and with a small subset of CD3+ve T cells. CD161 is a homodimeric cell surface protein comprising two chains of 40-44kD. Clone B199. 2 recognises a similar epitope to the DX9 monoclonal antibody.
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106cells in 100ul. Method sheets are available on request. NCBI
Gene ID:
3820
Isotype:
CD161
Clone:
B199. 2
Fusion Partner:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63. Ag8. 653 myeloma cell line.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Dilution:
Flow Cytometry - 1/50 - 1/100
Function:
Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1 3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
Subunit:
Homodimer; disulfide-linked. Interacts with acid sphingomyelinase/SMPD1.
Subcellular Location:
Membrane; Single-pass type II membrane protein (Potential).
Tissue Specificity:
Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
Induction:
By IL12 in NK cells.
Ptm:
N-glycosylated. Contains sialic acid residues.
Similarity:
Contains 1 C-type lectin domain. 1. Bennett. I. M. et al. (1996) Definition of natural killer NKR-PIA+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. 2. Azzoni. L. et al. (1998) Differential transcriptional regulation of CD161 and a novel gene. 197/15a. by IL-2. IL-15. and IL-12 in NK and T cells.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Discount: -10.0%
Price: €137.14
You save: €15.24
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