CD282

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone TL2. 1
Applications FC, IP, IHC
Amount 0.025 mg
ArtNr 20-783-310255
Available
Genway ID:
GWB-6C9FC0
Specificity:
CD282NCBI
Gene ID:
7097
Isotype:
IgG2a
Clone:
TL2. 1
Immunogen:
CHO-TLR2 cells.
Fusion Partner:
Spleen cells from immunised Balb/c mice were fused with cells of the NSO myeloma cell line.
Specificity Note:
This product recognises human TLR2 otherwise known as CD282. TLR2 is a member of the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes. TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. TLR2 is reported to respond to a diverse range of bacterial cell wall components mediating the innate immune response in co-operation with MD-2. Clone TL2. 1 is reported to block TLR2 function. Removal of sodium azide is recommended prior to use in functional studies. Histology: Tonsil
Buffer Solution:
Phosphate buffered saline
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - 1/10 - 1/50
Function:
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6 leading to NF-kappa-B activation cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2) soluble tuberculosis factor (STF) phenol-soluble modulin (PSM) and B. burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Subunit:
Interacts with LY96 TLR2 and TLR6 via the extracellular domain. Binds MYD88 via their respective TIR domains. Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1.
Subcellular Location:
Membrane; Single-pass type I membrane protein (By similarity).
Tissue Specificity:
Highly expressed in peripheral blood leukocytes in particular in monocytes in bone marrow lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Ptm:
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
Polymorphism:
Genetic variations in TLR2 are associated with suceptibility to leprosy [MIM:246300]. Leprosy is a chronic disease associated with depressed cellular (but not humoral) immunity the bacterium requires a lower temperature than 37 degrees Celsius and thrives particularly in peripheral Schwann cells and macrophages. The Trp-677 polymorphism in the intracellular domain of TLR2 has a role in susceptibility to lepromatous leprosy. Wild-type TLR2 mediates CD14-enhanced Mycobacterium leprae-dependent activation of NFKB1 but TLR2 containing the Trp-677 polymorphism did not. The impaired function of the Trp-677 polymorphism provides a molecular mechanism for the poor cellular immune response associated with lepromatous leprosy.
Similarity:
Belongs to the Toll-like receptor family.
Similarity:
Contains 14 LRR (leucine-rich) repeats.
Similarity:
Contains 1 TIR domain. 1. Lien. E. et al. (1999) Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. 2. Flo. T. H. et al. (2001) Differential expression of toll-like receptor 2 in human cells.
Amount: 0.025 mg
Available: In stock
Listprice: €212.88
Discount: -10.0%
Price: €191.59
You save: €21.29
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