CD13

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone R3-63
Applications FC
Amount 0.025 mg
ArtNr 20-783-310265
Available
Genway ID:
GWB-7CC18C
Specificity:
CD13NCBI
Gene ID:
16790
Specificity:
CD13
Clone:
R3-63
Immunogen:
Mouse intestinal APN
Fusion Partner:
Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium Azide1%Bovine Serum AlbuminSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (/). Suggested
Dilution:
Flow Cytometry - Neat - 1/5
Function:
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells macrophages granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Catalytic Activity:
Release of an N-terminal amino acid Xaa-|-Yaa- from a peptide amide or arylamide. Xaa is preferably Ala but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue the two may be released as an intact Xaa-Pro dipeptide. Cofactor: Binds 1 zinc ion per subunit (By similarity).
Subunit:
Homodimer. Interacts with the S1 domain of HCoV-229E spike protein.
Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Cytoplasm cytosol (Potential). Note=A soluble form has also been detected.
Tissue Specificity:
Expressed in epithelial cells of the kidney intestine and respiratory tract; granulocytes monocytes fibroblasts endothelial cells cerebral pericytes at the blood-brain barrier synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
Induction:
Estradiol and IL-8 decrease enzymatic activity in vitro in endometrial stromal cells by 40% and 30% respectively.
Domain:
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
Ptm:
Sulfated (By similarity).
Ptm:
N- and O-glycosylated.
Ptm:
May undergo proteolysis and give rise to a soluble form.
Disease:
Defects in ANPEP may be a cause of various types of leukemia or lymphoma.
Miscellaneous:
Found to serve as a receptor for tumor-homing peptides more specifically NGR peptides. It could serve thus as a target for delivering drugs into tumors. Concentration in human hepatic bile varies from 17. 3 to 57. 6 micrograms/ml.
Similarity:
Belongs to the peptidase M1 family [view classification]. 1. Hansen. A. S. et al. (1993) A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibilty complex class II molecules. 2. Larsen. S . L. et al. (1996) T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides.
Amount: 0.025 mg
Available: In stock
Listprice: €182.24
Discount: -10.0%
Price: €164.02
You save: €18.22
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