Antibodies

CD22

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone OX-96
Applications FC
Amount 25 TESTS
ArtNr 20-783-310270
Available
Genway ID:
GWB-B19FF4
Specificity:
CD22NCBI
Gene ID:
12483
Specificity:
CD22
Clone:
OX-96
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium Azide1%Bovine Serum Albumin Serum
Reconstitution: Reconstitute in 0. 25 ml disilled waterSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (/). Suggested
Dilution:
Flow Cytometry - Neat - 1/10
Function:
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2 6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Subunit:
Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1 LYN SYK PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2 INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP GRB2 and SHC1.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:
B-lymphocytes.
Domain:
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Ptm:
Phosphorylation of Tyr-762 Tyr-807 and Tyr-822 are involved in binding to SYK GRB2 and SYK respectively. Phosphorylation of Tyr-842 is involved in binding to SYK PLCG2 and PIK3R1/PIK3R2.
Ptm:
Phosphorylated on tyrosine residues by LYN (By similarity).
Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Similarity:
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Amount: 25 TESTS
Available: In stock
Listprice: €212.88
Price: €212.88
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