Antibodies

CD283

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone TLR3. 7
Applications WB, FC, IP
Amount 0.025 mg
ArtNr 20-783-310311
Available
Genway ID:
GWB-8CECCC
Specificity:
CD283NCBI
Gene ID:
7098
Isotype:
IgG1
Clone:
TLR3. 7
Immunogen:
Ba/F3 cells transfected with human TLR3 (CD283).
Fusion Partner:
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Specificity Note:
This product recognises human and canine TLR3 otherwise known as CD283. TLR3 is a member of the Toll-like receptor (TLR) family and functions as a receptor for double-stranded (ds) RNA. TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. Cell surface expression of TLR3 has been reported on MRC-5 fibroblasts (1) and this antibody has been shown to suppress poly (I) : poly C mediated IFN beta production by these cells.
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Membrane permeabilisation is required for this application. We recommend the use of Leucoperm for this purpose. Suggested
Dilution:
Flow Cytometry - 1/10 - 1/50Western Blotting - 1/100 - 1/1000
Function:
Participates in the innate immune response to microbial agents. Mediates the innate immune response to ds-RNA a sign of viral infection. Acts via MYD88 and TRAF6 leading to NF-kappa-B activation cytokine secretion and the inflammatory response.
Subunit:
Binds MYD88 via their respective TIR domains (By similarity). Interacts with TICAM1. Homodimer formation is triggered by ligand binding and is required for TLR3 signaling. Binding of ds-RNA is required for the interaction with SRC.
Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane.
Tissue Specificity:
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes including monocyte precursors.
Ptm:
Heavily N-glycosylated except on that part of the surface of the ectodomain that is involved in ligand binding.
Similarity:
Belongs to the Toll-like receptor family.
Similarity:
Contains 22 LRR (leucine-rich) repeats.
Similarity:
Contains 1 TIR domain. 1. Matsumoto. M. et al. (2002) Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling.
Amount: 0.025 mg
Available: In stock
Listprice: €212.88
Price: €212.88
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