CD18

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone M18/2
Applications WB, FC, IP
Amount 0.025 mg
ArtNr 20-783-310344
Available
Genway ID:
GWB-72C06D
Specificity:
CD18
Specificity:
CD18
Clone:
M18/2
Immunogen:
Membrane glycoproteins of cytotoxic T-cells
Fusion Partner:
Spleen cells from immunised Wistar - Furth rats were fused with cells of the mouse P3X63Ag8. 653 myeloma cell line.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - 1/100 - 1/200Western Blotting - Non-reducing conditions
Function:
Integrin alpha-L/beta-2 is a receptor for ICAM1 ICAM2 ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Subunit:
Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L alpha-M alpha-X or alpha-D. Interacts with COPS5 and RANBP9.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Ptm:
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Disease:
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type I (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Similarity:
Belongs to the integrin beta chain family.
Similarity:
Contains 1 VWFA domain. 1. Sanchez-Madrid. F. et al. (1983) Mapping of antigenic and functional epitopes on the alpha - and beta-subunits of two related mouse glycoproteins involved in cell interactions. LFA-1 and MAC-1. 2. Zahalka. M. A. et al. (1993) Blocking Lymphoma Invasiveness with a Monoclonal Antibody Directed against the Beta-Chain of the Leukocyte Adhesion Molecule (CD18). 3. Driessens. M. H. et al. (1996) Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1. -2 and -3 are distinct. J. Leukoc. Biol. 60:758-765.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Discount: -10.0%
Price: €137.14
You save: €15.24
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