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MAdCAM-1

MAdCAM-1


Manufacturer	GENWAY
Category	Oncology Antibodies Cell Biology Cell Movement Cell Adhesion Flow Cytometry ELISA Primary Antibodies By Organ Colorectal Cancer
Type	Antibody
Specific against	other
Clone	17FS
Applications	FC, ELISA
Amount	0.025 mg
Item no.	20-783-310378
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-3632E1
Specificity:	MAdCAM-1NCBI
Gene ID:	8174
Specificity:	MAdCAM-1
Clone:	17FS
Immunogen:	Recombinant soluble MAdCAM-1-fc fusion protein
Fusion Partner:	Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8. 653 myeloma cell line.
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline pH7. 4
Preservative Stabilisers:		0. 09%Sodium Azide1%Bovine Serum AlbuminSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:	Flow Cytometry - Neat - 1/10ELISA - 1ug/ml - 10ug/ml
Function:	Cell adhesion leukocyte receptor expressed by mucosal venules helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin regulating both the passage and retention of leukocytes. Isoform 2 lacking the mucin-like domain may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening independent of L-selectin binding.
Subunit:	Homodimer (Probable).
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Tissue Specificity:	Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus prostate ovaries testis heart placenta lung liver skeletal muscle kidneys or peripheral blood leukocytes.
Ptm:	The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation (By similarity).
Polymorphism:	The number of repeats in the mucin domain varies between 5 and 8 repeats.
Similarity:	Contains 2 Ig-like (immunoglobulin-like) domains. 1. Leung. E. et al. (1996) Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts 2. Leung. E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.

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Amount: 0.025 mg

available

Delivery expected until 5/9/2024