SIGN-R1

SIGN-R1

ER-TR9

Spleen cells from immunised rats were fused with cells of the Mouse P3-X63-Ag8. 563 myeloma cell line.

Phosphate buffered saline pH7. 4

Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested

Probable pathogen-recognition receptor. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. May recognize in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens. Is a receptor for ICAM3 probably by binding to mannose-like carbohydrates.

Expressed in skin spleen and lung probably in a subset of dendritic cells. Detected in spleen extrafollicular paracortical areas including the red pulp and marginal zones and at lower levels in the follicular area. Detected in skin suprabasal areas adjacent to the epidermis and in epidermal cell layer.

Contains 1 C-type lectin domain. 1. van Vliet. E. et al. (1985) Marginal zone macrophages in the mouse spleen identified by a monoclonal antibody. 2. Teunis. B. H. et al. (2002) Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo. 3. Kang. Y. S. et al. (2003) SIGN-R1. a novel C-type lectin expressed by marginal zone macrophages in spleen. mediates uptake of the polysaccharide dextran. 4. Kraal. G. et al. (1989) Marginal zone macrophages and their role in the immune response against T-independent type 2 antigens: modulation of the cells with specific antibody.