Antibodies

CD71

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone ER-MP21
Applications FC, IP
Amount 0.025 mg
ArtNr 20-783-310449
Available
Genway ID:
GWB-4F816E
Specificity:
CD71
Specificity:
CD71
Clone:
ER-MP21
Immunogen:
Balb/c macrophage precursor cell hybrids
Fusion Partner:
Cells from immunised rats were fused with cells of the rat Y3-Ag1. 2. 3 myeloma cell line.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - 1/50 - 1/100
Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand the heditary hemochromatosis protein HFE competes for binding with transferrin for an overlapping C-terminal binding site.
Subunit:
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen DR1. Interacts with SH3BP3.
Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Subcellular Location:
Transferrin receptor protein 1 serum form: Secreted.
Induction:
Regulated by cellular iron levels through binding of the iron regulatory proteins IRP1 and IRP2 to iron-responsive elements in the 3\' -UTR. Up-regulated upon mitogenic stimulation.
Ptm:
N- and O-glycosylated phosphorylated and palmitoylated. The serum form is only glycosylated.
Ptm:
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Ptm:
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Miscellaneous:
Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemia resulting from a number of conditions including rheumatoid arthritis pregnancy irritable bowel syndrome and in HIV patients.
Miscellaneous:
Canine and feline parvoviruses bind human and feline transferrin receptors and use these receptors to enter and infect cells.
Similarity:
Belongs to the peptidase M28 family. M28B subfamily [view classification].
Similarity:
Contains 1 PA (protease associated) domain. 1. Leenen. P. J. M. et al. (1990) Differential inhibition of macrophage proliferation by anti-transferrin receptor antibody ER-MP21: correlation to macrophage differentiation stage.
Amount: 0.025 mg
Available: In stock
Listprice: €177.85
Price: €177.85
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