Manufacturer |
GENWAY
|
Category |
|
Type |
Antibody |
Specific against |
other |
Clone |
PO3 |
Applications |
FC |
Amount |
25 TESTS |
Item no. |
20-783-310497 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Genway ID: |
GWB-55F153 |
Specificity: |
CD86NCBI |
Gene ID: |
12524 |
Specificity: |
CD86 |
Clone: |
PO3 |
Immunogen: |
Mouse B-cell line BCL1. |
Fusion Partner: |
Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution: |
Phosphate buffered saline pH7. 4 |
|
Preservative Stabilisers: |
| 0. 09%Sodium Azide1%Bovine Serum Albumin Reconstitution: Reconstitute in 0. 25 ml disilled waterSuggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested |
Dilution: |
Flow Cytometry - Neat - 1/10 |
Function: |
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters and thus acts as a negative regulator of T-cell activation. |
Subunit: |
Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses. |
Subcellular Location: |
Membrane; Single-pass type I membrane protein. |
Tissue Specificity: |
Expressed by activated B-lymphocytes and monocytes. |
Ptm: |
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
Similarity: |
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
Similarity: |
Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Jin. J. P. et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings. 2. Nozawa. K. et al. (2001) Preferential blockade of CD8+ T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases. 3. Nakajima. A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells. |
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