Antibodies

ZAP 70

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone ZAP-03
Applications WB, FC
Amount 0.025 mg
ArtNr 20-783-310553
Available
Genway ID:
GWB-19C901
Specificity:
ZAP 70NCBI
Gene ID:
7535
Specificity:
ZAP 70
Clone:
ZAP-03
Immunogen:
Bacterially expressed fusion protein corresponding to the C-terminal region of human Zap-70.
Preparation:
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium Azide (NaN3) Suggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Membrane permeabilisation is required for this application. We recommend the use of Leucoperm () for this purp
Function:
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction isoform 2 does not.
Catalytic Activity:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit:
Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity). Interacts with FCRL3.
Subcellular Location:
Cytoplasm (By similarity). Note=After antigen stimulation isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic (By similarity).
Tissue Specificity:
Expressed in T- and natural killer cells.
Domain:
The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
Ptm:
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Disease:
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Similarity:
Contains 1 protein kinase domain.
Similarity:
Contains 2 SH2 domains. 1. Chan. A. C. et al. (1991) The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70. a 70-kDa tyrosine phosphoprotein. 2. Orchard. J. et al. (2005) ZAP-70 in B cell malignancies.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Price: €152.38
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