CD169

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone 7-239
Applications FC
Amount 0.025 mg
ArtNr 20-783-310568
Available
Genway ID:
GWB-834779
Specificity:
CD169NCBI
Gene ID:
6614
Isotype:
IgG1
Clone:
7-239
Immunogen:
Human Rhinovirus (HRV14) treated monocyte-derived dendritic cells.
Fusion Partner:
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8. 653 myeloma cell line.
Specificity:
This poduct specifically recognises human CD169 otherwise known as Sialoadhesin (Sn) a type I transmembrane glycoprotein and prototypic member of the Siglec (sialic acid binding Ig-like lectin) family designated Siglec-1. CD169 is a macrophage-restricted interaction molecule which binds to sialylated ligands on haematopoietic cells including neutrophils NK cells monocytes a subset of CD8+ T cells and B cells through recognition of sialic acid in the alpha-2 3- glycosidic linkage. CD169 is highly expressed by dendritic cells and on stromal macrophages in the spleen bone marrow and lymph nodes and to a lesser extent in liver lungs and gut and studies have shown high expression of CD169 on macrophages in the inflammatory conditions rheumatoid arthritis and atherosclerosis. Clone 7-239 completely inhibits erythrocyte rosetting around Sn+ transductants. recommend the use of this product for use in functional studies.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline
Preservative Stabilisers:
0. 09%Sodium Azide (NaN3)1%Bovine Serum Albumin Suggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - Neat - 1/10
Function:
Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes including granulocytes monocytes natural killer cells B-cells and CD8 T-cells. Preferentially binds to alpha-2 3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein.
Subcellular Location:
Isoform 2: Secreted.
Tissue Specificity:
Expressed by macrophages in various tissues. High levels are found in spleen lymph node perivascular macrophages in brain and lower levels in bone marrow liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Similarity:
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Kirchberger. S. et al. (2005) Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. 2. Hartnell. A. et al. (2001) Characterization of human sialoadhesin. a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
Amount: 0.025 mg
Available: In stock
Listprice: €182.24
Discount: -10.0%
Price: €164.02
You save: €18.22
lieferbar

Get an offer

Technische Fragen zu diesem Produkt?


Salesmanager: Arne Pelz

Email: sales@hoelzel.de

Close