CD142


Manufacturer	GENWAY
Category	Antibodies Flow Cytometry Primary Antibodies
Type	Antibody
Specific against	other
Clone	CLB/TF-5
Applications	FC
Amount	0.025 mg
Item no.	20-783-310598
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-B4B83C
Specificity:	CD142
Isotype:	IgG1
Clone:	CLB/TF-5
Product Form:	Purified igG conjugated to Flourescein Isothiocyanate Isomer 1 (FITC) - liquid.
Specificity:	20-783-310598 recognises human CD142 a 45kD cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes macrophages and endothelial cells by various stimuli including interleukin 1 tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation angiogenesis and the pathophysiology of atherosclerosis and cancer. The CLB/TF-5 antibody has been used successfully in an ELISA assay as a coating antibody in association with clone CLB/TF-1 (20-783-310598) which recognises a different epitope of CD142.
Preparation:	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution:	TRIS buffered saline pH 8. 0
Preservative Stabilisers:		0. 09% Sodium Azide (NaN3) 1% Bovine Serum AlbuminSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Function:	Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Induction:	TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF as well as by endotoxin to appear on monocytes and vascular endothelial cells as a component of cellular immune response.
Similarity:	Belongs to the tissue factor family. 1. van der Putten. R. F. et al. (2005) High-affinity antibodies in a new immunoassay for plasma tissue factor: reduction in apparent intra-individual variation.

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Amount: 0.025 mg

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Delivery expected until 5/30/2024