CD4


Manufacturer	GENWAY
Category	Antibodies Flow Cytometry Primary Antibodies
Type	Antibody
Specific against	other
Clone	RM4-5
Applications	FC
Amount	0.025 mg
Item no.	20-783-310635
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-C4AA9C
Specificity:	CD4NCBI
Gene ID:	12504
Specificity:	CD4
Clone:	RM4-5
Immunogen:	BALB/c mouse thymocytes
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline pH7. 2
Preservative Stabilisers:		0. 09%Sodium Azide (NaN3)1%Bovine Serum Albumin Suggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (/).
Function:	Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Subunit:	Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins.
Subcellular Location:	Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with and sequesters CD4 in the endoplasmic reticulum.
Ptm:	Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Miscellaneous:	Primary receptor for HIV-1.
Similarity:	Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Similarity:	Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Fehervari. Z. and Sakaguchi. S. (2004) CD4+ Tregs and immune control.

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Amount: 0.025 mg

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Delivery expected until 5/9/2024