Manufacturer |
GENWAY
|
Category |
|
Type |
Antibody |
Specific against |
other |
Clone |
53-6. 7 |
Applications |
FC, IP, IHC |
Amount |
0.025 mg |
Item no. |
20-783-310648 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Genway ID: |
GWB-8CE70D |
Specificity: |
CD8 ALPHANCBI |
Gene ID: |
12525 |
Specificity: |
CD8 ALPHA |
Clone: |
53-6. 7 |
Immunogen: |
Mouse thymus or spleen. |
Immunohistology: |
This product has been reported as working well on Zinc-fixed paraffin-embedded sections. However. inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution: |
Phosphate buffered saline pH7. 2 |
|
Preservative Stabilisers: |
| 0. 09%Sodium Azide (NaN3) Suggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (/). |
Function: |
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
Subunit: |
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. |
Subcellular Location: |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Subcellular Location: |
Isoform 2: Secreted. |
Ptm: |
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers while the four N-terminal cysteines do not (By similarity). |
Disease: |
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells leading to recurrent bacterial infections. |
Similarity: |
Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Jabbari. A. and Harty. J. T. (2006) The generation and modulation of antigen-specific memory CD8 T cell responses. 2. Takahashi. K. et al. (1992) CD4 and CD8 regulate interleukin 2 responses of T cells. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.