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CD8 ALPHA

CD8 ALPHA


Manufacturer	GENWAY
Category	Oncology Antibodies Flow Cytometry Immunohistochemistry Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody
Specific against	other
Clone	KT15
Applications	FC, IHC
Amount	0.025 mg
Item no.	20-783-310700
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-A2421D
Specificity:	CD8 ALPHANCBI
Gene ID:	12525
Specificity:	CD8 ALPHA
Clone:	KT15
Immunogen:	T cell clone C6
Fusion Partner:	Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
Preparation:	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline pH7. 4
Preservative Stabilisers:		0. 09%Sodium AzideSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:	Flow Cytometry - 1/50 - 1/100
Function:	Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Subunit:	In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.
Subcellular Location:	Isoform 1: Cell membrane; Single-pass type I membrane protein.
Subcellular Location:	Isoform 2: Secreted.
Ptm:	All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers while the four N-terminal cysteines do not (By similarity).
Disease:	Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells leading to recurrent bacterial infections.
Similarity:	Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Tomonari. K. et al. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11 positive mouse T-cell clone. 2. Whiteland. J. L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.

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Amount: 0.025 mg

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Delivery expected until 5/30/2024